A biochemical network controlling basal myosin oscillation

Qin, Xiang and Hannezo, Edouard and Mangeat, Thomas and Liu, Chang and Majumder, Pralay and Liu, Jiaying and Choesmel-Cadamuro, Valerie and McDonald, Jocelyn A and Liu, Yinyao and Yi, Bin and Wang, Xiaobo (2018) A biochemical network controlling basal myosin oscillation. Nature Communications, 9 (1). Article number: 1210 . ISSN 2041-1723

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Official URL: http://dx.doi.org/10.1038/s41467-018-03574-5

Abstract

The actomyosin cytoskeleton, a key stress-producing unit in epithelial cells, oscillates spontaneously in a wide variety of systems. Although much of the signal cascade regulating myosin activity has been characterized, the origin of such oscillatory behavior is still unclear. Here, we show that basal myosin II oscillation in Drosophila ovarian epithelium is not controlled by actomyosin cortical tension, but instead relies on a biochemical oscillator involving ROCK and myosin phosphatase. Key to this oscillation is a diffusive ROCK flow, linking junctional Rho1 to medial actomyosin cortex, and dynamically maintained by a self-activation loop reliant on ROCK kinase activity. In response to the resulting myosin II recruitment, myosin phosphatase is locally enriched and shuts off ROCK and myosin II signals. Coupling Drosophila genetics, live imaging, modeling, and optogenetics, we uncover an intrinsic biochemical oscillator at the core of myosin II regulatory network, shedding light on the spatio-temporal dynamics of force generation.

Item Type: Article
DOI: 10.1038/s41467-018-03574-5
Uncontrolled Keywords: Myosin, morphogenesis, computational biophysics
Subjects: 500 Science > 530 Physics > 539 Modern physics
500 Science > 570 Life sciences; biology
Research Group: Hannezo Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 05 Apr 2018 09:51
Last Modified: 05 Apr 2018 09:51
URI: https://repository.ist.ac.at/id/eprint/996

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