A micro-CT-based method for quantitative brain lesion characterization and electrode localization

Masís, Javier and Mankus, David and Wolff, Steffen B and Guitchounts, Grigori and Joesch, Maximilian and Cox, David D (2018) A micro-CT-based method for quantitative brain lesion characterization and electrode localization. Scientific Reports, 8 (1). Article Number: 5184 . ISSN 20452322

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Official URL: http://dx.doi.org/10.1038/s41598-018-23247-z

Abstract

Lesion verification and quantification is traditionally done via histological examination of sectioned brains, a time-consuming process that relies heavily on manual estimation. Such methods are particularly problematic in posterior cortical regions (e.g. visual cortex), where sectioning leads to significant damage and distortion of tissue. Even more challenging is the post hoc localization of micro-electrodes, which relies on the same techniques, suffers from similar drawbacks and requires even higher precision. Here, we propose a new, simple method for quantitative lesion characterization and electrode localization that is less labor-intensive and yields more detailed results than conventional methods. We leverage staining techniques standard in electron microscopy with the use of commodity micro-CT imaging. We stain whole rat and zebra finch brains in osmium tetroxide, embed these in resin and scan entire brains in a micro-CT machine. The scans result in 3D reconstructions of the brains with section thickness dependent on sample size (12-15 and 5-6 microns for rat and zebra finch respectively) that can be segmented manually or automatically. Because the method captures the entire intact brain volume, comparisons within and across studies are more tractable, and the extent of lesions and electrodes may be studied with higher accuracy than with current methods.

Item Type: Article
DOI: 10.1038/s41598-018-23247-z
Uncontrolled Keywords: Neuroscience, sensorimotor processing
Subjects: 500 Science > 570 Life sciences; biology > 571 Physiology
500 Science > 570 Life sciences; biology > 572 Biochemistry
Research Group: Jösch Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 03 Apr 2018 11:38
Last Modified: 03 Apr 2018 11:38
URI: https://repository.ist.ac.at/id/eprint/994

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