Real time analysis of auxin response cell wall pH and elongation in Arabidopsis thaliana Hypocotyls

Li, Lanxin and Krens, Gabriel and Fendrych, Matyas and Friml, Jiří (2018) Real time analysis of auxin response cell wall pH and elongation in Arabidopsis thaliana Hypocotyls. Bio-protocol, 8 (1). Article number: e2685 . ISSN 2331-8325

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Official URL: http://dx.doi.org/10.21769/BioProtoc.2685

Abstract

The rapid auxin-triggered growth of the Arabidopsis hypocotyls involves the nuclear TIR1/AFB-Aux/IAA signaling and is accompanied by acidification of the apoplast and cell walls (Fendrych et al., 2016). Here, we describe in detail the method for analysis of the elongation and the TIR1/AFB-Aux/IAA-dependent auxin response in hypocotyl segments as well as the determination of relative values of the cell wall pH.

Item Type: Article
DOI: 10.21769/BioProtoc.2685
Uncontrolled Keywords: Auxin signaling, cell wall pH, cell elongation, Hypocotyl, live-cell imaging
Subjects: 500 Science > 570 Life sciences; biology > 576 Genetics and evolution
500 Science > 580 Plants > 581 Botany
Research Group: Friml Group
Heisenberg Group
Scientific Service Units > Bioimaging
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 15 Feb 2018 08:22
Last Modified: 15 Feb 2018 08:22
URI: https://repository.ist.ac.at/id/eprint/970

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