WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity

Prat, Tomas and Hajny, Jakub and Grunewald, Wim and Vasileva, Mina and Molnar, Gergely and Tejos, Ricardo and Schmid, Markus and Sauer, Michael and Friml, Jiří (2018) WRKY23 is a component of the transcriptional network mediating auxin feedback on PIN polarity. PLoS Genetics, 14 (1). Article number: e1007177. ISSN 1553-7404

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Abstract

Auxin is unique among plant hormones due to its directional transport that is mediated by the polarly distributed PIN auxin transporters at the plasma membrane. The canalization hypothesis proposes that the auxin feedback on its polar flow is a crucial, plant-specific mechanism mediating multiple self-organizing developmental processes. Here, we used the auxin effect on the PIN polar localization in Arabidopsis thaliana roots as a proxy for the auxin feedback on the PIN polarity during canalization. We performed microarray experiments to find regulators of this process that act downstream of auxin. We identified genes that were transcriptionally regulated by auxin in an AXR3/IAA17- and ARF7/ARF19-dependent manner. Besides the known components of the PIN polarity, such as PID and PIP5K kinases, a number of potential new regulators were detected, among which the WRKY23 transcription factor, which was characterized in more detail. Gain- and loss-of-function mutants confirmed a role for WRKY23 in mediating the auxin effect on the PIN polarity. Accordingly, processes requiring auxin-mediated PIN polarity rearrangements, such as vascular tissue development during leaf venation, showed a higher WRKY23 expression and required the WRKY23 activity. Our results provide initial insights into the auxin transcriptional network acting upstream of PIN polarization and, potentially, canalization-mediated plant development.

Item Type: Article
DOI: 10.1371/journal.pgen.1007177
Subjects: 500 Science > 580 Plants > 581 Botany
Research Group: Friml Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 12 Feb 2018 13:50
Last Modified: 12 Feb 2018 13:50
URI: https://repository.ist.ac.at/id/eprint/967

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