A phytochrome sensory domain permits receptor activation by red light

Reichhart, Eva and Ingles-Prieto, Alvaro and Tichy, Alexandra-Madelaine and McKenzie, Catherine and Janovjak, Harald (2016) A phytochrome sensory domain permits receptor activation by red light. Angewandte Chemie - International Edition, 55 (21). pp. 6339-6342. ISSN 1521-3773

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Official URL: http://dx.doi.org/10.1002/anie.201601736

Abstract

Optogenetics and photopharmacology enable the spatio-temporal control of cell and animal behavior by light. Although red light offers deep-tissue penetration and minimal phototoxicity, very few red-light-sensitive optogenetic methods are currently available. We have now developed a red-light-induced homodimerization domain. We first showed that an optimized sensory domain of the cyanobacterial phytochrome 1 can be expressed robustly and without cytotoxicity in human cells. We then applied this domain to induce the dimerization of two receptor tyrosine kinases—the fibroblast growth factor receptor 1 and the neurotrophin receptor trkB. This new optogenetic method was then used to activate the MAPK/ERK pathway non-invasively in mammalian tissue and in multicolor cell-signaling experiments. The light-controlled dimerizer and red-light-activated receptor tyrosine kinases will prove useful to regulate a variety of cellular processes with light. Go deep with red: The sensory domain (S) of the cyanobacterial phytochrome 1 (CPH1) was repurposed to induce the homodimerization of proteins in living cells by red light. By using this domain, light-activated protein kinases were engineered that can be activated orthogonally from many fluorescent proteins and through mammalian tissue. Pr/Pfr=red-/far-red-absorbing state of CPH1.

Item Type: Article
DOI: 10.1002/anie.201601736
Uncontrolled Keywords: Optogenetics, receptors, photochromism, phytochrome
Subjects: 500 Science > 570 Life sciences; biology > 571 Physiology
500 Science > 570 Life sciences; biology > 576 Genetics and evolution
Research Group: Janovjak Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 03 Jul 2017 15:10
Last Modified: 05 Sep 2017 09:41
URI: https://repository.ist.ac.at/id/eprint/840

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