Optical control of ligand-gated ion channels

Szobota, Stephanie and McKenzie, Catherine and Janovjak, Harald (2013) Optical control of ligand-gated ion channels. Methods in Molecular Biology, 998. pp. 417-435. ISSN 1064-3745

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Official URL: http://dx.doi.org/10.1007/978-1-62703-351-0_32

Abstract

In the vibrant field of optogenetics, optics and genetic targeting are combined to commandeer cellular functions, such as the neuronal action potential, by optically stimulating light-sensitive ion channels expressed in the cell membrane. One broadly applicable manifestation of this approach are covalently attached photochromic tethered ligands (PTLs) that allow activating ligand-gated ion channels with outstanding spatial and temporal resolution. Here, we describe all steps towards the successful development and application of PTL-gated ion channels in cell lines and primary cells. The basis for these experiments forms a combination of molecular modeling, genetic engineering, cell culture, and electrophysiology. The light-gated glutamate receptor (LiGluR), which consists of the PTL-functionalized GluK2 receptor, serves as a model.

Item Type: Article
DOI: 10.1007/978-1-62703-351-0_32
Uncontrolled Keywords: Optical switch, azobenzene, glutamate receptor, optogenetics, optochemical genetics, photochromic tethered ligand, LiGluR
Subjects: 500 Science > 570 Life sciences; biology > 571 Physiology
500 Science > 570 Life sciences; biology > 576 Genetics and evolution
Research Group: Janovjak Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 03 Jul 2017 13:51
Last Modified: 05 Sep 2017 14:38
URI: https://repository.ist.ac.at/id/eprint/834

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