Srv2/CAP is required for polarized actin cable assembly and patch internalization during clathrin-mediated endocytosis

Toshima, Junko Y and Horikomi, Chika and Okada, Asuka and Hatori, Makiko N and Nagano, Makoto and Masuda, Atsushi and Yamamoto, Wataru and Siekhaus, Daria E and Toshimaa, Jiro (2016) Srv2/CAP is required for polarized actin cable assembly and patch internalization during clathrin-mediated endocytosis. Journal of Cell Science, 129. pp. 367-379. ISSN 1477-9137

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Official URL: http://dx.doi.org/10.1242/jcs.176651

Abstract

The dynamic assembly and disassembly of actin filaments is essential for the formation and transport of vesicles during endocytosis. In yeast, two types of actin structures, namely cortical patches and cytoplasmic cables, play a direct role in endocytosis, but how their interaction is regulated remains unclear. Here, we show that Srv2/CAP, an evolutionarily conserved actin regulator, is required for efficient endocytosis owing to its role in the formation of the actin patches that aid initial vesicle invagination and of the actin cables that these move along. Deletion of the SRV2 gene resulted in the appearance of aberrant fragmented actin cables that frequently moved past actin patches, the sites of endocytosis. We find that the C-terminal CARP domain of Srv2p is vitally important for the proper assembly of actin patches and cables; we also demonstrate that the N-terminal helical folded domain of Srv2 is required for its localization to actin patches, specifically to the ADP-actin rich region through an interaction with cofilin. These results demonstrate the in vivo roles of Srv2p in the regulation of the actin cytoskeleton during clathrin-mediated endocytosis

Item Type: Article
DOI: 10.1242/jcs.176651
Uncontrolled Keywords: Endocytosis, actin, Srv2, CAP, Cofilin
Subjects: 500 Science > 570 Life sciences; biology
500 Science > 570 Life sciences; biology > 576 Genetics and evolution
Research Group: Siekhaus Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 23 Feb 2017 06:55
Last Modified: 05 Sep 2017 09:54
URI: https://repository.ist.ac.at/id/eprint/767

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