Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells

Łangowski, Łukasz and Wabnik, Krzysztof and Li, Hongjiang and Vanneste, Steffen and Naramoto, Satoshi and Tanaka, Hirokazu and Friml, Jiří (2016) Cellular mechanisms for cargo delivery and polarity maintenance at different polar domains in plant cells. Cell Discovery, 2. Article number: 1601 . ISSN 2056-5968

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Abstract

The asymmetric localization of proteins in the plasma membrane domains of eukaryotic cells is a fundamental manifestation of cell polarity that is central to multicellular organization and developmental patterning. In plants, the mechanisms underlying the polar localization of cargo proteins are still largely unknown and appear to be fundamentally distinct from those operating in mammals. Here, we present a systematic, quantitative comparative analysis of the polar delivery and subcellular localization of proteins that characterize distinct polar plasma membrane domains in plant cells. The combination of microscopic analyses and computational modeling revealed a mechanistic framework common to diverse polar cargos and underlying the establishment and maintenance of apical, basal, and lateral polar domains in plant cells. This mechanism depends on the polar secretion, constitutive endocytic recycling, and restricted lateral diffusion of cargos within the plasma membrane. Moreover, our observations suggest that polar cargo distribution involves the individual protein potential to form clusters within the plasma membrane and interact with the extracellular matrix. Our observations provide insights into the shared cellular mechanisms of polar cargo delivery and polarity maintenance in plant cells.

Item Type: Article
DOI: 10.1038/celldisc.2016.18
Subjects: 500 Science > 580 Plants
Research Group: Friml Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 20 Feb 2017 08:32
Last Modified: 05 Sep 2017 10:04
URI: https://repository.ist.ac.at/id/eprint/757

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