Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation

Sako, Keisuke and Pradhan, Saurabh J and Barone, Vanessa and Ingles-Prieto, Alvaro and Mueller, Patrick and Ruprecht, Verena and Capek, Daniel and Galande, Sanjeev and Janovjak, Harald and Heisenberg, Carl-Philipp (2016) Optogenetic control of nodal signaling reveals a temporal pattern of nodal signaling regulating cell fate specification during gastrulation. Cell Reports, 16 (3). pp. 866-877. ISSN 2211-1247

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Official URL: http://dx.doi.org/10.1016/j.celrep.2016.06.036

Abstract

During metazoan development, the temporal pattern of morphogen signaling is critical for organizing cell fates in space and time. Yet, tools for temporally controlling morphogen signaling within the embryo are still scarce. Here, we developed a photoactivatable Nodal receptor to determine how the temporal pattern of Nodal signaling affects cell fate specification during zebrafish gastrulation. By using this receptor to manipulate the duration of Nodal signaling in vivo by light, we show that extended Nodal signaling within the organizer promotes prechordal plate specification and suppresses endoderm differentiation. Endoderm differentiation is suppressed by extended Nodal signaling inducing expression of the transcriptional repressor goosecoid (gsc) in prechordal plate progenitors, which in turn restrains Nodal signaling from upregulating the endoderm differentiation gene sox17 within these cells. Thus, optogenetic manipulation of Nodal signaling identifies a critical role of Nodal signaling duration for organizer cell fate specification during gastrulation.

Item Type: Article
DOI: 10.1016/j.celrep.2016.06.036
Subjects: 500 Science > 570 Life sciences; biology
500 Science > 570 Life sciences; biology > 576 Genetics and evolution
Research Group: Heisenberg Group
Janovjak Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 15 Feb 2017 07:47
Last Modified: 05 Sep 2017 09:46
URI: https://repository.ist.ac.at/id/eprint/754

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