Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein

Friml, Jiří and Michalko, Jaroslav and Glanc, Matous and Perrot-Rechenmann, Catherine (2016) Strong morphological defects in conditional Arabidopsis abp1 knock-down mutants generated in absence of functional ABP1 protein. F1000Research, 5. Article number: 86 . ISSN 2046-1402

[img] Text
770cf1e0-612f-4e85-a500-54b6349fbbab_7654_-_jaroslav_michalko.pdf - Published Version
Available under License Creative Commons Attribution.
[IST-2016-711-v1+1]
Download (2920Kb)
Official URL: http://dx.doi.org/10.12688/f1000research.7654.1

Abstract

The Auxin Binding Protein 1 (ABP1) is one of the most studied proteins in plants. Since decades ago, it has been the prime receptor candidate for the plant hormone auxin with a plethora of described functions in auxin signaling and development. The developmental importance of ABP1 has recently been questioned by identification of Arabidopsis thaliana abp1 knock-out alleles that show no obvious phenotypes under normal growth conditions. In this study, we examined the contradiction between the normal growth and development of the abp1 knock-outs and the strong morphological defects observed in three different ethanol-inducible abp1 knock-down mutants ( abp1-AS, SS12K, SS12S). By analyzing segregating populations of abp1 knock-out vs. abp1 knock-down crosses we show that the strong morphological defects that were believed to be the result of conditional down-regulation of ABP1 can be reproduced also in the absence of the functional ABP1 protein. This data suggests that the phenotypes in abp1 knock-down lines are due to the off-target effects and asks for further reflections on the biological function of ABP1 or alternative explanations for the missing phenotypic defects in the abp1 loss-of-function alleles.

Item Type: Article
DOI: 10.12688/f1000research.7654.1
Subjects: 500 Science > 580 Plants > 581 Botany
Research Group: Friml Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 06 Dec 2016 13:35
Last Modified: 04 Sep 2017 14:54
URI: https://repository.ist.ac.at/id/eprint/711

Actions (login required)

View Item View Item