Down syndrome cell adhesion molecule 1: Testing for a role in insect immunity, behaviour and reproduction

Peuß, Robert and Wensing, Kristina U and Woestmann, Luisa and Eggert, Hendrik and Milutinovic, Barbara and Sroka, Marlene G U and Scharsack, Jörn P and Kurtz, Joachim and Armitage, Sophie A (2016) Down syndrome cell adhesion molecule 1: Testing for a role in insect immunity, behaviour and reproduction. Royal Society Open Science, 3 (4). Article number: 160138 . ISSN 2054-5703

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Official URL: http://dx.doi.org/10.1098/rsos.160138

Abstract

Down syndrome cell adhesion molecule 1 (Dscam1) has widereaching and vital neuronal functions although the role it plays in insect and crustacean immunity is less well understood. In this study, we combine different approaches to understand the roles that Dscam1 plays in fitness-related contexts in two model insect species. Contrary to our expectations, we found no short-term modulation of Dscam1 gene expression after haemocoelic or oral bacterial exposure in Tribolium castaneum, or after haemocoelic bacterial exposure in Drosophila melanogaster. Furthermore, RNAi-mediated Dscam1 knockdown and subsequent bacterial exposure did not reduce T. castaneum survival. However, Dscam1 knockdown in larvae resulted in adult locomotion defects, as well as dramatically reduced fecundity in males and females. We suggest that Dscam1 does not always play a straightforward role in immunity, but strongly influences behaviour and fecundity. This study takes a step towards understanding more about the role of this intriguing gene from different phenotypic perspectives.

Item Type: Article
DOI: 10.1098/rsos.160138
Uncontrolled Keywords: Drosophila melanogaster, Locomotion, Dscam1, Fecundity, Immune defence, Tribolium castaneum
Subjects: 500 Science > 570 Life sciences; biology > 576 Genetics and evolution
500 Science > 590 Animals (zoology) > 592 Invertebrates
Research Group: Cremer Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 06 Dec 2016 09:49
Last Modified: 30 Aug 2017 11:52
URI: https://repository.ist.ac.at/id/eprint/704

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