Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis

Mazur, Ewa and Benková, Eva and Friml, Jiří (2016) Vascular cambium regeneration and vessel formation in wounded inflorescence stems of Arabidopsis. Scientific Reports, 6. Article number: 33754 . ISSN 20452322

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Synchronized tissue polarization during regeneration or de novo vascular tissue formation is a plant-specific example of intercellular communication and coordinated development. According to the canalization hypothesis, the plant hormone auxin serves as polarizing signal that mediates directional channel formation underlying the spatio-temporal vasculature patterning. A necessary part of canalization is a positive feedback between auxin signaling and polarity of the intercellular auxin flow. The cellular and molecular mechanisms of this process are still poorly understood, not the least, because of a lack of a suitable model system. We show that the main genetic model plant, Arabidopsis (Arabidopsis thaliana) can be used to study the canalization during vascular cambium regeneration and new vasculature formation. We monitored localized auxin responses, directional auxin-transport channels formation, and establishment of new vascular cambium polarity during regenerative processes after stem wounding. The increased auxin response above and around the wound preceded the formation of PIN1 auxin transporter-marked channels from the primarily homogenous tissue and the transient, gradual changes in PIN1 localization preceded the polarity of newly formed vascular tissue. Thus, Arabidopsis is a useful model for studies of coordinated tissue polarization and vasculature formation after wounding allowing for genetic and mechanistic dissection of the canalization hypothesis.

Item Type: Article
DOI: 10.1038/srep33754
Subjects: 500 Science > 580 Plants > 581 Botany
Research Group: Benkova Group
Friml Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 05 Dec 2016 14:57
Last Modified: 05 Sep 2017 09:27

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