Mosaic analysis with double markers reveals cell type specific paternal growth dominance

Hippenmeyer, Simon and Johnson, Randy L and Luo, Liqun (2013) Mosaic analysis with double markers reveals cell type specific paternal growth dominance. Cell Reports, 3 (3). pp. 960-967. ISSN 2211-1247

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Abstract

Genomic imprinting leads to preferred expression of either the maternal or paternal alleles of a subset of genes. Imprinting is essential for mammalian development, and its deregulation causes many diseases. However, the functional relevance of imprinting at the cellular level is poorly understood for most imprinted genes. We used mosaic analysis with double markers (MADM) in mice to create uniparental disomies (UPDs) and to visualize imprinting effects with single-cell resolution. Although chromosome 12 UPD did not produce detectable phenotypes, chromosome 7 UPD caused highly significant paternal growth dominance in the liver and lung, but not in the brain or heart. A single gene on chromosome 7, encoding the secreted insulin-like growth factor 2 (IGF2), accounts for most of the paternal dominance effect. Mosaic analyses implied additional imprinted loci on chromosome 7 acting cell autonomously to transmit the IGF2 signal. Our study reveals chromosome- and cell-type specificity of genomic imprinting effects.

Item Type: Article
DOI: 10.1016/j.celrep.2013.02.002
Subjects: 500 Science > 570 Life sciences; biology
Research Group: Hippenmeyer Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 04 Jan 2016 14:11
Last Modified: 05 Sep 2017 14:25
URI: https://repository.ist.ac.at/id/eprint/405

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