Control of directed cell migration in vivo by membrane-to-cortex attachment

Diz-Muñoz, Alba and Krieg, Michael and Bergert, Martin and Ibarlucea-Benitez, Itziar and Müller, Daniel J and Paluch, Ewa and Heisenberg, Carl-Philipp (2010) Control of directed cell migration in vivo by membrane-to-cortex attachment. PLoS Biology, 8 (11). Article No. e1000544. ISSN 1545-7885

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Cell shape and motility are primarily controlled by cellular mechanics. The attachment of the plasma membrane to the underlying actomyosin cortex has been proposed to be important for cellular processes involving membrane deformation. However, little is known about the actual function of membrane-to-cortex attachment (MCA) in cell protrusion formation and migration, in particular in the context of the developing embryo. Here, we use a multidisciplinary approach to study MCA in zebrafish mesoderm and endoderm (mesendoderm) germ layer progenitor cells, which migrate using a combination of different protrusion types, namely, lamellipodia, filopodia, and blebs, during zebrafish gastrulation. By interfering with the activity of molecules linking the cortex to the membrane and measuring resulting changes in MCA by atomic force microscopy, we show that reducing MCA in mesendoderm progenitors increases the proportion of cellular blebs and reduces the directionality of cell migration. We propose that MCA is a key parameter controlling the relative proportions of different cell protrusion types in mesendoderm progenitors, and thus is key in controlling directed migration during gastrulation.

Item Type: Article
Subjects: 500 Science > 570 Life sciences; biology > 576 Genetics and evolution
Research Group: Heisenberg Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 21 Dec 2015 07:47
Last Modified: 19 Sep 2017 15:32

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