BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana

Kitakura, Saeko and Adamowski, Maciej and Matsuura, Yuki and Santuari, Luca and Kouno, Hirotaka and Arima, Kohei and Hardtke, Christian S and Friml, Jiří and Kakimoto, Tatsuo and Tanaka, Hirokazu (2017) BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. Plant and Cell Physiology. Article number: pcx118. ISSN 1471-9053

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Official URL: http://dx.doi.org/10.1093/pcp/pcx118

Abstract

Membrane traffic at the trans-Golgi network (TGN) is crucial for correctly distributing various membrane proteins to their destination. Polarly localized auxin efflux proteins, including PIN-FORMED1 (PIN1), are dynamically transported between the endosomes and the plasma membrane (PM) in the plant cells. The intracellular trafficking of PIN1 protein is sensitive to a fungal toxin brefeldin A (BFA), which is known to inhibit guanine-nucleotide exchange factors for ADP ribosylation factors (ARF GEFs) such as GNOM. However, the molecular details of the BFA-sensitive trafficking pathway have not been revealed fully. In a previous study, we have identified an Arabidopsis mutant BFA-visualized endocytic trafficking defective 3 (ben3) which exhibited reduced sensitivity to BFA in terms of BFA-induced intracellular PIN1 agglomeration. Here, we show that BEN3 encodes a member of BIG family ARF GEFs, BIG2. Fluorescent proteins tagged BEN3/BIG2 co-localized with markers for TGN / early endosome (EE). Inspection of conditionally induced de novo synthesized PIN1 confirmed that its secretion to the PM is BFA-sensitive and established BEN3/BIG2 as a crucial component of this BFA action at the level of TGN/EE. Furthermore, ben3 mutation alleviated BFA-induced agglomeration of another TGN-localized ARF GEF BEN1/MIN7. Taken together our results suggest that BEN3/BIG2 is an ARF GEF component, which confers BFA sensitivity to the TGN/EE in Arabidopsis.

Item Type: Article
DOI: 10.1093/pcp/pcx118
Additional Information: This is a pre-copyedited, author-produced PDF of an article accepted for publication in Plant and Cell Physiology following peer review. The version of record Kitakura, Saeko, Adamowski, Maciek, Matsuura, Yuki, Santuari, Luca, Kouno, Hirotaka, Arima, Kohei, Hardtke, Christian S, Friml, Jiří, Kakimoto, Tatsuo & Tanaka, Hirokazu, 2017. BEN3/BIG2 ARF GEF is Involved in Brefeldin A-Sensitive Trafficking at the trans-Golgi Network/Early Endosome in Arabidopsis thaliana. Plant and Cell Physiology, Article number: pcx118- is available online at http://dx.doi.org/10.1093/pcp/pcx118.
Uncontrolled Keywords: Arabidopsis, Auxin, trans-Golgi Network, Brefeldin A, ARF GEF, PIN-FORMED1
Subjects: 500 Science > 580 Plants > 581 Botany
Research Group: Friml Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 16 Apr 2018 12:54
Last Modified: 16 Apr 2018 12:57
URI: https://repository.ist.ac.at/id/eprint/1009

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