Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development

Ugalde, José M and Rodríguez-Furlán, Cecilia and De Rycke, Riet M U and Norambuena, Lorena and Friml, Jiří and León, Gabriel and Tejos, Ricardo (2016) Phosphatidylinositol 4-phosphate 5-kinases 1 and 2 are involved in the regulation of vacuole morphology during Arabidopsis thaliana pollen development. Plant Science, 250. pp. 10-19. ISSN 0168-9452

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Official URL: http://dx.doi.org/10.1016/j.plantsci.2016.05.014

Abstract

The pollen grains arise after meiosis of pollen mother cells within the anthers. A series of complex structural changes follows, generating mature pollen grains capable of performing the double fertilization of the female megasporophyte. Several signaling molecules, including hormones and lipids, have been involved in the regulation and appropriate control of pollen development. Phosphatidylinositol 4-phophate 5-kinases (PIP5K), which catalyze the biosynthesis of the phosphoinositide PtdIns(4,5)P2, are important for tip polar growth of root hairs and pollen tubes, embryo development, vegetative plant growth, and responses to the environment. Here, we report a role of PIP5Ks during microgametogenesis. PIP5K1 and PIP5K2 are expressed during early stages of pollen development and their transcriptional activity respond to auxin in pollen grains. Early male gametophytic lethality to certain grade was observed in both pip5k1-/- and pip5k2-/- single mutants. The number of pip5k mutant alleles is directly related to the frequency of aborted pollen grains suggesting the two genes are involved in the same function. Indeed PIP5K1 and PIP5K2 are functionally redundant since homozygous double mutants did not render viable pollen grains. The loss of function of PIP5K1 and PIP5K2results in defects in vacuole morphology in pollen at the later stages and epidermal root cells. Our results show that PIP5K1, PIP5K2 and phosphoinositide signaling are important cues for early developmental stages and vacuole formation during microgametogenesis.

Item Type: Article
DOI: 10.1016/j.plantsci.2016.05.014
Additional Information: Author's post-print must be released with a Creative Commons Attribution Non-Commercial No Derivatives License.
Uncontrolled Keywords: Vacuole, Microgametogenesis, Phosphoinositides, PIP5K, Pollen development
Subjects: 500 Science > 580 Plants > 581 Botany
Research Group: Friml Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 16 Apr 2018 12:13
Last Modified: 16 Apr 2018 12:35
URI: https://repository.ist.ac.at/id/eprint/1005

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