PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis

Simon, Sibu and Skůpa, Petr and Viaene, Tom and Zwiewka, Marta and Tejos, Ricardo and Klíma, Petr and Čarná, Mária and Rolčík, Jakub and De Rycke, Riet M U and Moreno, Ignacio and Dobrev, Petre Ivanov and Orellana, Ariel and Zažímalová, Eva and Friml, Jiří (2016) PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist, 211 (1). pp. 65-74. ISSN 1469-8137

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Official URL: http://dx.doi.org/10.1111/nph.14019

Abstract

Plant development mediated by the phytohormone auxin depends on tightly controlled cellular auxin levels at its target tissue that are largely established by intercellular and intracellular auxin transport mediated by PIN auxin transporters. Among the eight members of the Arabidopsis PIN family, PIN6 is the least characterized candidate. In this study we generated functional, fluorescent protein-tagged PIN6 proteins and performed comprehensive analysis of their subcellular localization and also performed a detailed functional characterization of PIN6 and its developmental roles. The localization study of PIN6 revealed a dual localization at the plasma membrane (PM) and endoplasmic reticulum (ER). Transport and metabolic profiling assays in cultured cells and Arabidopsis strongly suggest that PIN6 mediates both auxin transport across the PM and intracellular auxin homeostasis, including the regulation of free auxin and auxin conjugates levels. As evidenced by the loss- and gain-of-function analysis, the complex function of PIN6 in auxin transport and homeostasis is required for auxin distribution during lateral and adventitious root organogenesis and for progression of these developmental processes. These results illustrate a unique position of PIN6 within the family of PIN auxin transporters and further add complexity to the developmentally crucial process of auxin transport.

Item Type: Article
DOI: 10.1111/nph.14019
Additional Information: This is the peer reviewed version of the following article: Simon, Sibu, Skůpa, Petr, Viaene, Tom, Zwiewka, Marta, Tejos, Ricardo, Klíma, Petr, Čarná, Mária, Rolčík, Jakub, De Rycke, Riet M, Moreno, Ignacio, Dobrev, Petre Ivanov, Orellana, Ariel, Zažímalová, Eva & Friml, Jiří, 2016. PIN6 auxin transporter at endoplasmic reticulum and plasma membrane mediates auxin homeostasis and organogenesis in Arabidopsis. New Phytologist, 211(1), 65-74, which has been published in final form at http://dx.doi.org/10.1111/nph.14019. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.
Uncontrolled Keywords: Lateral root, Auxin, PIN, Endoplasmic reticulum (ER), Plasma membrane (PM)
Subjects: 500 Science > 580 Plants > 581 Botany
Research Group: Friml Group
SWORD Depositor: Sword Import User
Depositing User: Sword Import User
Date Deposited: 16 Apr 2018 11:59
Last Modified: 16 Apr 2018 12:21
URI: https://repository.ist.ac.at/id/eprint/1004

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